Table 1.

Primers used in the polymerase chain reaction (PCR) screen of endogenous Banana streak Goldfinger virus (eBSGFV).

Primer name	Primer sequence (5′–3′)*	Expected product size (bp)
VM1‐F†	TTGTCCAAAATCTGCTCGTG	481
VM1‐R	TGTAATTCCTGCTCCTGCAA
VM2‐F†	TTCTCCCTTTTCGATCCGTA	374
VM2‐R	TTTTGATGCATCTCCAGCAG
VV1‐F	ACAGCTCCAGGAGATTGGAA	268
VV1‐R	CTGAAGTGTGCCTGTGGAGA
VV2‐F	TCTGAGATCTCCAGCCAGGT	639
VV2‐R	GACAGTTCCAGCACAGCAGA
VV3‐F	TTGCCAAGAATTCCTCCAAG	376
VV3‐R	AAGTTCTTGTCGGCAAGGTG
VV4‐F	GAGCAACACGAGTCAACGAA	784
VV4‐R	TCTCCACAGGCACACTTCAG
VV5‐F‡	CCATGGAGGTTGACCTGTCT	588
VV5‐R	ACCCCTCTGTCTTCCCAACT
VV5bis‐F§	CGCACCTTCATCACAGAAGA	628
VV5bis‐R	TACCAGATGGGGAGAAATCG
VV6‐F	GCATGAAGCATGACTGGAGA	264
VV6‐R	AATGCATAAGGGCCTCGAAT

^* Primer annealing temperature: 60 °C.

^† Primer pairs ‘VM’ amplify the junction between the Musa genome and endogenous Banana streak virus (eBSV); primer pairs ‘VV’ amplify the internal fragment junctions within eBSGFV.

^‡ Primer pairs characterize eBSGFV‐9.

^§ Primer pairs external to primer ‡ on the same DNA fragment.