Figure 4.

In vitro Bamboo mosaic virus (BaMV) RNA template activity assay‐based competition assay of Ba‐77 mutants. (A) Autoradiograph of BaMV RNA template activity assay with Ba‐77 alone (lanes 1, 4, 7, and 10) or in the presence of competitors Ba‐39 (lanes 2 and 3), Ba‐77/Δ31 (lanes 5 and 6) and yeast tRNA (lanes 8 and 9) at the different molar excesses indicated above each lane. Control with RNA‐dependent RNA polymerase (RdRp) alone is shown in lane 11 or Ba‐77 without RdRp in the reaction is shown in lane 12. (B) The signals in (A) were quantified and plotted as the percentage of the levels of Ba‐77 in the absence of any competitors. The data presented are the average and error bars of at least three independent reactions.