Table 1.

The levels of accumulation of viral products of Bamboo mosaic virus (BaMV) and its derivatives in protoplasts of Nicotiana benthamiana leaves.

Mutant	Genomic RNA*	Genomic RNA†	Minus‐strand RNA‡	Plus/minus§
BaMV40A	1	1	1	1
BaMVΔ1A	0.91 ± 0.24	0.95 ± 0.25	0.93 ± 0.25	1.02
BaMVΔ2A	0.02 ± 0.01	0.03 ± 0.02	0.05 ± 0.03	0.6
BaMVΔ3A	0.01 ± 0.00	0.01 ± 0.01	0.01 ± 0.01	ND¶
BaMVΔ4A	0.01 ± 0.01	0.01 ± 0.01	0.02 ± 0.01	ND
BaMVA2C	0.07 ± 0.05	0.10 ± 0.03	0.13 ± 0.03	0.77
BaMVA2U	0.07 ± 0.02	0.09 ± 0.02	0.14 ± 0.07	0.64
BaMVA2G	0.40 ± 0.12	0.45 ± 0.01	0.60 ± 0.01	0.75

^* Genomic RNA accumulation level in protoplasts, detected by Northern blotting with a probe complementary to 0.6 kb of the 3′‐end of BaMV RNA. The level of wild‐type BaMV40A genomic RNA was set at unity. All data are the average (± standard deviation) of at least three independent experiments.

^† Genomic RNA accumulation level in protoplasts, detected by quantitative real‐time reverse transcriptase‐polymerase chain reaction (qRT‐PCR) with TaqMan probe.

^‡ Minus‐strand genomic RNA accumulation level in protoplasts, detected by qRT‐PCR with SYBR green I dye.

^§ The plus/minus value results from the levels of genomic RNA over the minus‐strand RNA, both measured by qRT‐PCR.

^¶ ND, not determined. The level of plus‐ and minus‐strand RNA accumulation is too low to be counted effectively.