Table 1.

Appressorium formation of the chs7 ⁻mutant and wild‐type (WT) in the presence and absence of different surfaces and inducers.

Strain	Hydrophobic No inducer	Hydrophobic 1,16‐HDD	Hydrophobic 8‐CPT‐cAMP	Hydrophobic DOG	Onion epidermis (untreated)	Onion epidermis (treated)
70‐15	99.7 ± 0.58	99.3 ± 1.4	99.7 ± 0.58	98.33 ± 0.6	88.4 ± 3.5	83.9 ± 5.8
chs7	0	80.3 ± 2.1	71.0 ± 3.61	77.67 ± 3.1	70.7 ± 1.7	0

Spores were incubated at 10⁵ conidia/mL for 24 h on the surface indicated. The percentages of spores that formed one or more appressoria are shown. No appressoria were formed by either strain on a hydrophilic surface (glass microscope slides). The data shown are the mean values obtained by counting 300 spores for each strain, and the standard deviation is given. The experiment was repeated three times with very similar results. The inducers used were the cutin monomer 1,16‐hexadecanediol (1,16‐HDD; 200 ng/mL), the diacylglycerol 1,2‐dioctanoyl‐rac‐glycerol (DOG; 20 µg/mL) and the cyclic adenosine monophosphate (cAMP) analogue 8‐(4‐chlorophenylthio)‐cAMP (8‐CPT‐cAMP; 40 µg/mL).