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. 2010 Nov 25;12(4):365–372. doi: 10.1111/j.1364-3703.2010.00677.x

Figure 1.

Figure 1

Plasmids used in this study. (A) Cloning plasmids containing the chiA gene cloned from either maize inbred LH82 (pTAN114) or B73 (pTAN112; not shown). The exon–intron borders are shown below; the amino acids encoded by the exons are displayed above the DNA sequence with the amino acid letter above the first letter of the codon. (B) Expression plasmids. The two chiA cDNAs were cloned into pPICZαA, creating pTAN121 (LH82 allele) and pTAN122 (B73 allele; not shown). The fusion of the yeast secretion signal sequence to the chiA cDNA is shown below; an EcoRI restriction endonuclease site connects the secretion signal to the ChitA‐encoding chiA sequence; the Ste13 protease of Pichia pastoris removes the secretion signal during secretion from the yeast.