Table 2.

Plasmids used in this study.

Plasmid name	Description
pBluescript KS(+) cloning plasmids
pTAN112	chiA gene from maize inbred B73
pTAN114*	chiA gene from maize inbred LH82
pTAN112ΔI	chiA cDNA from maize inbred B73
pTAN114ΔI	chiA cDNA from maize inbred LH82
pTAN112ΔI(A47D)	chiA from B73 with A47D mutation
pTAN114ΔI(D48A)	chiA from LH82 with D48A mutation
pPICZαA expression plasmids
pTAN121*	Expression of rChitA‐LH82
pTAN122	Expression of rChitA‐B73
pTAN121(D48A)	Expression of rChitA‐LH82 with D48A mutation
pTAN122(A47D)	Expression of rChitA‐B73 with A47D mutation

^* Plasmids shown in Fig. 1.