Proposed secondary structure of minimum free energy of CEVdCOL. Discontinuous lines divide the secondary structure into the five structural domains (Terminal left, TL; Pathogenicity, P; Central, C; Variable, V; and Terminal right, TR). Conserved regions [central conserved region (CCR) and terminal conserved region (TCR)] are shaded in the TL and C domains. PL and PR are boxed in the P and V domains. Boxed nucleotides (square boxes) show nucleotide changes found in CEVdCOL when compared with the severe variant CEVdE‐117. Boxed nucleotide (round box) shows the nucleotide change found in the CEVd variants recovered from inoculated tomatoes.