Table 5.

Primers used in reverse transcriptase‐polymerase chain reaction (RT‐PCR) designed on six genes found to be differentially regulated by microarray experiment.

GenBank #	Annotation	Corresponding clone on TIGR array	Primer name	Primer sequences 5'–3'
P13046	Pathogenesis‐related protein R major form precursor (thaumatin‐like protein E22)	STMIR63 STMIT96	P8	Forward: ATGGGCCAGGGTCATGTA Reverse: GCCTTCAAGGGCAAAAGA
Q8S3U2	EDS1‐like protein	STMIX37	P10	Forward: CTTCTGCACTGGGAATAGGA Reverse: TTCGGAACTCAGTTGAGAGG
Q9FZ08	Patatin‐like protein 3	STMJP54	P13	Forward: TGGTATAGGCAAGCAGGAAG Reverse: TATCAAAGGAGTGGCACCAT
AAF09479	Phytoalexin‐deficient 4 protein (PAD4)	STMEN29	P11	Forward: TTGCATTACCTTTGGCTCTC Reverse: CATGATGGGGAAAAGAACAG
Q84V46	Alternative oxidase 1b (AOX)	STMIP26	P14	Forward: GGTTGGATCAAAGCCCTACT Reverse: ATGCACGTTCGTACCATTTT
Q9AXU5	Pathogen‐inducible α‐dioxygenase (PIOX)	STMCZ55 STMEZ47	P15	Forward: ACAATGGGATGGAAGTGCTA Reverse: GTTCAAGGAGCCCATTTTCT

EDS1, enhanced disease susceptibility 1; TIGR, Institute of Genomic Research.