Figure 5.

Transcriptional analysis of defence‐related genes in tomato plants inoculated with the pathogen Botrytis cinerea and pretreated or not with Trichoderma spp. The relative expression of PR1b1, PR‐P2, PINI, PINII, TomLoxA and TomLoxC was measured by quantitative reverse transcription real‐time polymerase chain reaction (qRT‐PCR) at different times after pathogen infection [0, 24 and 48 h post‐inoculation (hpi)] in 2‐month‐old plants of Solanum lycopersicum (Corbarino, TA209, M82 and SM36) and S. habrochaites (LA1777) lines, developed from untreated (cont), T. atroviride P1‐treated (P1) or T. harzianum T22‐treated (T22) seeds. The expression levels for each gene are reported as the fold increase relative to that of the control plants not treated with Trichoderma before infection with the pathogen (0 hpi), in a semi‐logarithmic scale. Values within the dotted lines were not considered to be significantly different from the control. Data on PINII expression in the wild tomato line LA1777 are missing as no amplification of this gene could be obtained with the primer pair used. The results shown are from one representative experiment out of four.