Figure 4.

FLS2 and RIN4 may reside in the same protein complex. The pulldown assays were performed using solubilized membrane proteins [either without or with dithiobis(succinimidyl propionate) (DSP) cross‐linking] prepared from 5‐week‐old Arabidopsis plants of FLS2::HPB fls2 (FLS2::HPB) and fls2 (control). FLS2::HPB was pulled down with streptavidin beads. The pulldown and input samples were examined by immunoblotting with anti‐haemagglutinin (HA) and anti‐RIN4 antibodies. This experiment was performed three times with similar results.