Table 3.
Principal disease resistance, defence and stress response genes up‐regulated by Xac and Xaa at 6 and 48 hpi, relative to water‐infiltrated leaves. Genes identified by DD/SSH not represented in the citrus array are shown in bold and their expression levels were determined by qRT‐PCR. The complete list of disease resistance, defence and stress response genes with altered expression upon Xac/Xaa infection is found in Supporting Information Tables S1, S8 and S9.
| Target description | Citrus | Target | Change after treatments | |||||
|---|---|---|---|---|---|---|---|---|
| Microarray | DD/SSH | |||||||
| 6 hpi | 48 hpi | 48 hpi | ||||||
| Disease resistance, defence and stress response | EST | Gene ID | Xaa | Xac | Xaa | Xac | Xaa | Xac |
| Disease resistance protein [A. thaliana] | CX301461 | NP_199715 | 8.7 | 8.7 | 19.0 | 15.3 | — | — |
| Leucine‐rich repeat protein [A. thaliana] | CX642531 | NP_172219 | — | — | 6.6 | 6.5 | — | — |
| Resistance protein RPP8‐like protein [A. thaliana] | CX669576 | AAP82824 | 14.0 | 7.3 | 5.2 | 4.7 | — | — |
| Receptor protein kinase [A. thaliana] | CV707423 | AAO42089 | 13.4 | 9.5 | 7.6 | 7.3 | — | — |
| EIX receptor 2 [L. esculentum] | CX043514 | AAR28378 | — | — | 4.4 | 6.3 | — | — |
| LRR receptor like kinase [A. thaliana] | — | AK221860 | — | — | — | — | 11.8 | 14.7 |
| Disease resistance protein [A. thaliana] | — | AC020579 | — | — | — | — | 3.8 | 5.6 |
| BON1‐associated protein BAP1 [A. thaliana] | CX077288 | NP_182100 | 11.6 | 19.7 | 6.1 | — | — | — |
| WRKY‐type DNA binding protein [A. thaliana] | CX050828 | BAC42556 | 13.9 | 6.4 | 7.5 | 15.5 | — | — |
| WRKY‐type DNA binding protein [A. thaliana] | CF828414 | BAC23031 | — | — | 24.7 | 7.5 | — | — |
| Zinc finger (C3HC4‐type RING finger) [A. thaliana] | BQ623333 | NP_565376 | 3.1 | 6.1 | — | — | — | — |
| RING‐H2 zinc finger protein ATL3 [A. thaliana] | CX050623 | AAD33581 | 4.8 | 4.7 | 7.7 | 4.0 | — | — |
| ZPT2‐12 [Petunia × hybrida] | CK939354 | BAA21921 | 7.4 | 9.2 | — | — | — | — |
| F‐box family protein [A. thaliana] | CK935545 | NP_566322 | 3.4 | 3.4 | 7.4 | 4.9 | — | — |
| Calcium‐binding EF hand protein [A. thaliana] | CK939355 | NP_568568 | 3.7 | 10.0 | — | — | — | — |
| NAC domain protein NAC2 [A. thaliana] | CK940145 | AAM63330 | 11.3 | 4.5 | 39.0 | 7.8 | — | — |
| HSR203J like protein [C. chinense] | CN190145 | BAD11070 | — | — | 13.5 | 6.4 | — | — |
| Bacterial‐induced peroxidase [G. hirsutum] | CX078551 | AAD43561 | 45.1 | 41.7 | 36.3 | 28.4 | — | — |
| Peroxidase [P. nigra] | CF832245 | BAA11853 | 14.6 | 7.0 | 9.5 | 7.5 | — | — |
| Anionic peroxidase H [Z. mays] | CV718839 | AAC79953 | 19.1 | 5.9 | 8.3 | 3.5 | — | — |
| Apoplastic anionic gaiacol peroxidase [G. hirsutum] | CF835282 | AAL92037 | 36.7 | 13.6 | 14.1 | 6.7 | — | — |
| Peroxidase [P. balsamifera] | CV707821 | CAA66037 | 12.0 | 4.6 | 9.4 | 3.8 | — | — |
| Putative NADH‐dehydrogenase [P. sativum] | CX293755 | AAO27256 | 9.7 | 5.9 | 13.6 | 20.8 | — | — |
| Ttropinone reductase [A. thaliana] | CX078323 | NP_180480 | 38.3 | 21.6 | — | — | — | — |
| Tropinone reductase I [S. tuberosum] | CN191155 | CAC34420 | 11.9 | 12.9 | 26.7 | 10.3 | — | — |
| Glutathione S‐transferase [C. maxima] | CX297758 | BAC21263 | 48.2 | 32.6 | 18.0 | 21.2 | — | — |
| Respiratory burst oxidase [N. benthamiana] | CX301580 | BAC56865 | 14.0 | 4.3 | 12.1 | 11.3 | — | — |
| Alternative oxidase [P. tremula × P. tremuloides] | DN795132 | CAB64356 | 4.6 | 3.5 | 17.3 | 3.2 | — | — |
| Cytochrome P450 monooxygenase [A. thaliana] | CX636220 | AAM61746 | 10.1 | 5.7 | — | — | — | — |
| Cytochrome P450 [C. sinensis] | CX639356 | AAL24049 | 7.3 | 8.8 | 43.7 | 5.7 | — | — |
| Cytochrome P450 79A1 [S. vulgare] | CX296809 | Q43135 | 23.9 | 13.3 | 13.6 | 6.9 | — | — |
| Cytochrome P450 CYP79A2 [A. thaliana] | CB322199 | NP_568153 | 21.6 | 11.6 | 17.3 | 8.3 | — | — |
| Cytochrome P450 [A. thaliana] | CX301598 | BAB09969 | 25.3 | 11.3 | 19.2 | 8.2 | — | — |
| Cytochrome P450 like_TBP [N. tabacum] | CB322204 | D64052 | — | — | — | — | 4.2 | 2.9 |
| Lipase class 3 family protein [A. thaliana] | CN190043 | NP_973975 | 30.4 | 5.0 | 79.3 | 15.8 | — | — |
| Lipoxygenase LOX2 [P. deltoids] | CX671317 | DQ131179 | — | — | — | — | 8.0 | 4.4 |
| Phospholipase D gamma 1 [A. thaliana] | DN618160 | NP_192922 | — | — | 8.0 | 5.1 | 18.9 | 9.2 |
| Pathogenesis‐related protein PR‐1 [C. annuum] | CF653559 | AAK30143 | 7.0 | 4.2 | 22.7 | 25.3 | 187 | 233 |
| Pathogenesis‐related protein 5‐1 [H. annuus] | CF836158 | AAM21199 | 39.7 | 6.2 | 11.7 | 10.1 | — | — |
| Pathogenesis‐related protein 4b [C. chinense] | CX643738 | BAD11073 | 128.7 | 13.7 | 40.7 | 6.0 | — | — |
| Pathogenesis‐related protein 4A [N. tabacum] | CX637285 | CAA41437 | 12.2 | 4.1 | 27.6 | 14.0 | — | — |
| Phenylalanine‐ammonia lyase [Citrus sp.] | CX643181 | CAB42793 | 12.0 | 7.9 | 21.2 | 19.5 | — | — |
| Lectin‐like protein kinase [A. thaliana] | CX669884 | BAB09808 | 11.0 | 3.8 | 39.7 | 6.3 | — | — |
| Chitinase CHI1 [C. sinensis] | CX292066 | AAC35981 | 3.5 | 3.2 | 13.4 | 24.1 | — | — |
| Chitinase [H. brasiliensis] | CX671223 | CAA09110 | — | — | 7.6 | 7.7 | — | — |
| Chitinase [G. hirsutum] | CX637693 | CAA92277 | 18.5 | 3.1 | 26.3 | 13.8 | — | — |
| Leucoanthocyanidin dioxygenase [A. thaliana] | CX641508 | BAB02603 | 9.7 | 6.3 | 37.2 | 12.4 | — | — |
| Phytocyanin homolog [P. taeda] | CF836493 | AAF75824 | 42.7 | 6.9 | — | — | — | — |
| Dicyanin [L. esculentum] | CX297166 | AAF66242 | 7.0 | 3.1 | 27.2 | 24.5 | — | — |
| Patatin‐like protein 1 [N. tabacum] | CX295316 | AAF98368 | 11.0 | 4.1 | — | — | — | — |
| Ionotropic glutamate receptor GLR4 [O. sativa] | CV710376 | XP_478449 | 4.3 | 4.5 | 5.1 | 4.3 | — | — |
| Wts2L [C. lanatus] | DN620336 | BAA89230 | 4.9 | 5.2 | 5.1 | 3.3 | — | — |
| CPRD2 [V. unguiculata] | CX291051 | BAB33033 | 55.6 | 9.0 | 74.4 | 46.3 | — | — |
| Nectarin 5 [N. langsdorffii × N. sanderae] | CX299253 | AAP30841 | 84.65 | 18.65 | 123.7 | 106.7 | — | — |