Table 5.
Main disease resistance and defence‐related genes specifically regulated by Xaa, relative to water treatments. The expression levels of genes identified by DD/SSH were determined by qRT‐PCR. Negative numbers represent down‐regulation. The complete list of defence‐related genes specifically modulated by Xaa is found in Supporting Information Tables S6 and S7. Fold change values of Xac versus Xaa treatments are shown for comparison.
| Target description | Citrus | Target | Change after treatment | |||||
|---|---|---|---|---|---|---|---|---|
| Microarray | DD/SSH | |||||||
| 6 hpi | 48 hpi | 48 hpi | ||||||
| Disease resistance, defence and stress response | EST | Gene ID | Xaa | Xac/Xaa | Xaa | Xac/Xaa | Xaa | Xac |
| WRKY‐type DNA binding protein [A. thaliana] | CX046706 | AAP21232 | 3.3 | –2.2 | 4.0 | — | — | — |
| WRKY transcription factor 30 [V. aestivalis] | CX044130 | AAR92477 | 3.6 | — | — | — | — | — |
| WRKY transcription factor [C. annuum] | CX643787 | AAW66459 | — | — | 9.4 | –3.5 | — | — |
| SPF1 protein [I. batatas] | CX294239 | BAA06278 | 3.3 | — | 4.8 | — | — | — |
| MYB family transcription factor (MYB63) [A. thaliana] | CN188989 | NP_178039 | 3.6 | –2.2 | 4.0 | –2.0 | — | — |
| No apical meristem (NAM) family protein [A. thaliana] | CN191283 | NP_187057 | 3.4 | –2.4 | 6.5 | –2.2 | — | — |
| Ubiquitin [L. esculentum] | CX296271 | CAA51679 | 3.1 | — | 3.3 | — | — | — |
| Ubiquitin‐conjugating enzyme E2 [O. sativa] | CX298761 | XP_466921 | — | — | 5.2 | –3.2 | — | — |
| F‐box family protein [A. thaliana] | DN623568 | AAN31083 | — | — | 5.0 | — | — | — |
| F‐box family protein [A. thaliana] | CF509222 | AAN18072 | — | — | 3.6 | –2.2 | — | — |
| Zinc finger (C3HC4‐type RING finger) (BRH1) [A. thaliana] | CN190502 | NP_191705 | — | — | 3.5 | — | — | — |
| Zinc finger (C3HC4‐type RING finger) [A. thaliana] | CX287180 | NP_567222 | — | — | 3.4 | –2.0 | — | — |
| RING/C3HC4/PHD zinc finger‐like protein [C. melo] | CN186687 | AAO45753 | — | — | 4.9 | –2.0 | — | — |
| Hin1 protein—common tobacco [N. tabacum] | CB293794 | T03265 | — | — | 3.7 | — | — | — |
| Disease resistance protein (CC‐NBS‐LRR) [A. thaliana] | CX544709 | NP_195056 | — | — | 3.5 | — | — | — |
| Putative protein kinase Xa21 [O. sativa] | CN190501 | NP_918567 | 4.0 | — | — | — | — | — |
| MAP kinase [A. thaliana] | CX073386 | BAA04866 | 3.1 | — | — | — | — | — |
| Putative receptor‐like protein kinase 4 RLK4 [A. thaliana] | AJ489090 | BAC42412 | 4.2 | –2.6 | — | — | — | — |
| Serine/threonine protein kinase isolog [A. thaliana] | CV717931 | AAM20520 | 3.8 | — | 6.3 | –2.6 | — | — |
| Serine/threonine kinase [O. sativa] | CX640171 | BAD53292 | — | — | 3.0 | — | — | — |
| Receptor kinase 1 [A. thaliana] | CX292287 | BAB02668 | — | — | 3.3 | –2.4 | — | — |
| Protein kinase [A. thaliana] | CX302153 | NP_177852 | 4.8 | –2.4 | 5.6 | –2.7 | — | — |
| Mitogen‐activated protein kinase kinase MKK9 [A. thaliana] | CB610685 | NP_177492 | — | — | 3.3 | — | — | — |
| Receptor‐like protein kinase [N. tabacum] | CX297031 | BAC07504 | — | — | 4.0 | — | — | — |
| Protein kinase‐like protein [A. thaliana] | CX297218 | CAB62310 | — | — | 10.6 | –4.2 | — | — |
| Respiratory burst oxidase 2 [M. truncatula] | CX309380 | AAW78864 | — | — | 7.4 | — | — | — |
| FAD‐linked oxidase protein [A. thaliana] | CX302002 | AAQ56827 | 26.4 | –14.2 | 20.8 | –14.0 | — | — |
| Glycolate oxidase [M. crystallinum] | CK935794 | AAB40396 | 3.3 | –2.0 | 6.7 | –2.3 | — | — |
| CER1‐like protein [A. thaliana] | CV704769 | AAC24373 | 4.0 | — | 5.2 | — | — | — |
| Peroxidase [N. tabacum] | CV884382 | AAK52084 | — | — | 6.1 | –2.3 | — | — |
| Glutathione S‐transferase [E. esula] | CX669862 | AAF72197 | — | — | 4.2 | — | — | — |
| Hemoglobin [C. glauca] | CX636043 | CAA37898 | — | — | 9.9 | –4.2 | — | — |
| Thioredoxin family protein [A. thaliana] | CN186629 | NP_200952 | 4.7 | –2.2 | — | — | — | — |
| Proline oxidase/dehydrogenase 1 [N. tabacum] | CX544362 | AAT57674 | 6.0 | –2.5 | — | — | — | — |
| Proline dehydrogenase [G. max] | CK934047 | AAR86686 | 9.2 | –3.8 | 10.5 | –3.8 | — | — |
| Aldehyde dehydrogenase 1 precursor [L. corniculatus] | CX297720 | AAO72532 | 4.1 | –2.3 | 3.6 | — | — | — |
| NADH glutamate dehydrogenase [V. vinifera] | CX306249 | CAC18730 | 4.3 | –2.8 | 11.7 | –4.3 | — | — |
| Leucoanthocyanidin dioxygenase‐like protein [A. thaliana] | CX674852 | AAM91495 | 8.5 | –5.2 | 4.5 | — | — | — |
| Syringolide‐induced protein 1‐3‐1A [G. max] | CN189156 | BAB86892 | 3.7 | — | 3.8 | — | — | — |
| Ferulate 5‐hydroxylase [C. acuminata] | CF417783 | AAT39511 | 4.7 | — | 3.1 | — | — | — |
| N‐hydroxylating cytochrome P450 [M. esculenta] | CN191734 | AAF27289 | 7.4 | –2.4 | 5.3 | — | — | — |
| Cytochrome P450 [H. tuberosus] | CF504933 | CAA04117 | — | — | 3.7 | — | — | — |
| Cytochrome P450 [P. communis] | CX644731 | AAL66194 | — | — | 3.5 | — | — | — |
| Cytochrome P450 [A. majus] | CX308646 | AAS90126 | — | — | 5.4 | –2.2 | — | |
| Cytochrome P450 protein [A. thaliana] | CF509035 | AAM14385 | — | — | 4.6 | –2.0 | — | — |
| Cytochrome P450 [A. officinalis] | CX668706 | BAB40324 | — | –3.9 | 11.5 | –10.2 | — | — |
| Germin‐like protein [A. thaliana] | CN186507 | P92997 | — | — | 26.9 | –19.7 | — | — |
| Nodulin26‐like intrinsic protein [P. sativum] | DN958192 | CAB45652 | 4.9 | –2.0 | — | — | — | — |
| MTD1 [M. truncatula] | CX669382 | AAF86687 | 6.5 | –3.8 | — | — | — | — |
| Putative lipase [A. thaliana] | DN621566 | NP_568327 | 3.6 | — | 4.0 | — | — | — |
| 12‐oxophytodienoate reductase OPR2 [A. thaliana] | CX043279 | BAC42387 | 6.0 | –5.3 | 10.9 | –6.0 | — | — |
| Putative phosphatase [L. esculentum] | CX544543 | CAD30864 | 4.7 | –2.1 | 7.8 | –2.9 | — | — |
| Putative phosphatase [L. esculentum] | CX070476 | CAD30863 | 5.5 | –2.2 | 8.0 | –3.5 | 19.8 | 3.0 |
| Cysteine endopeptidase, papain‐type (XCP2) [A. thaliana] | CK935239 | NP_564126 | –3.3 | — | –3.3 | — | — | — |
| Chalcone synthase 2 [C. sinensis] | CF833966 | Q9XJ57 | –3.2 | — | –3.6 | — | — | — |
| Aquaporin [R. communis] | BQ623782 | CAE53882 | –4.4 | — | — | — | — | — |