Table 2.
Oligonucleotide primer | Nucleotide sequence (5′–3′)* |
---|---|
mucD_fwd | CGAATTTCTCGAGCGCAGCATGC |
mucD_revT7 | TAATACGACTCACTATAGGGAGGGGAGCGGGTAAATATCTGCG |
cmaA_fwd | TTTGAGTCGGTCTGCACGCA |
cmaA_revT7 | TAATACGACTCACTATAGGGAGGGCTGTACGTTGTCTACTAG |
cfl_fwd | ATGAGTCTGATTTCTGAGTTCCGCA |
cfl_revT7 | TAATACGACTCACTATAGGGAGGTAGTTATTCCTGTGGTGC |
corR_fwd | ATGCCGAGCTCTTCGATCTTGC |
corR_revT7 | TAATACGACTCACTATAGGGAGGAGTATCGCCTGGACATGG |
corS_fwd | AATACGGCGCGCTGTCAGTT |
corS_revT7 | TAATACGACTCACTATAGGGAGGAATGGATGGCCTAATAGGCG |
corP_fwd | ATCACGCCTTGTTCCGTT |
corP_revT7 | TAATACGACTCACTATAGGGAGGTCATTTGCAAATCGAGCAAGATGAGATCG |
23SrRNA_fwd#1 | ACGTGGACCAGCCCTTAAGTTGTATTG |
23SrRNA_revT7#1 | TAATACGACTCACTATAGGGAGGCCCCCATATTCAGACAAG |
23SrRNA_fwd#2 | CTTGAGTTCCCTGAAGGGCCGTCGAAG |
23SrRNA_revT7#2 | TAATACGACTCACTATAGGGAGGTGGTCAAGCCTCACGGGC |
lsc_fwd | GTCAGTGCGGACTTTCCGGTCATG |
lsc_revT7 | TAATACGACTCACTATAGGGAGGGATCGCGAAAGTTCCAGCTC |
T7 RNA polymerase promoter sequences incorporated in primers are shown in italics.