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. 2011 Jan 5;12(5):449–461. doi: 10.1111/j.1364-3703.2010.00683.x

Figure 6.

Figure 6

Morphological abnormalities observed in snt2 mutants of Fusarium oxysporum f.sp. melonis (FOM). Comparative analysis between Evans blue‐stained mycelial cells of the wild‐type isolate (A) and snt2 mutant strains detected decreased viability of the cells in mutants D122 (B) and Δsnt2 (C) of FOM (areas marked by circles). Monodansylcadaverine (MDC) staining of autophagosomes detected significant differences between the wild‐type (D) and the mutant isolates D122 (E) and Δsnt2 (F). Formation of autophagosomes in the presence of phenylmethanesulphonylfluoride (PMSF) and stained by MDC in the wild‐type isolate (G), D122 (H) and Δsnt2 (I) mutant isolates. Elimination of MDC staining in PMSF‐treated wild‐type cells (J) after addition of the specific autophagosome inhibitor 3‐methyladenine (K). Bars in A–C indicate 50 µm, whereas those in D–K indicate 10 µm.