Table 2.

Expression profile of nitrogen metabolism‐related genes in the Δsnt2 mutant of Fusarium oxysporum f.sp. melonis (FOM).

Gene	Accession number	Wild‐type/Δsnt2 expression ratio*	Description
idi4 †	FOXG_04081	24.5 ± 2.25+	Induced during incompatibility
cpc1	FOXG_05954	2.12 ± 0.09	Cross‐pathway control
pdc †	BAE98181.1	20.78 ± 7.04+	Pyruvate decarboxylase
fnr1	DQ387858	1.2 ± 0.15	Global nitrogen regulator
nit4	FOXG_06396	0.95 ± 0.08	Pathway‐specific nitrogen regulator
glnA	FOXG_05182	4.02 ± 0.50	Glutamine synthetase
mfs1 †	FOXG_09760	1.60 ± 0.86	Transporter of the major facilitator family
rbs1 †	FOXG_14252	0.15 ± 0.03‐	Unknown functions
eEF1G †	FOXG_01492	2.72 ± 1.21	Translation elongation factor

^* cDNA was synthesized from 1 µg of total RNA. All samples were analysed in triplicate. Averaged crossing point values were normalized to the endogenous control gene β‐tubulin. Gene expression level and comparative analyses (*) of two biological and four technical repeats were evaluated using the REST© program (Pfaffl et al., 2002). Accession numbers were according to http://www.broad.mit.edu and National Center for Biotechnology Information (NCBI) databases. Statistically significant changes are marked by += up‐regulated and −= down‐regulated.

^† Identified by suppressive subtraction hybridization (SSH) screen.