Figure 2.
Expression of the polyketide synthase (PKS)‐encoding genes revealed by reverse‐Northern analysis. Polymerase chain reaction (PCR) fragments of BcPKS genes predicted by Kroken et al. (2003) and other Botrytis cinerea genes were spotted onto Nylon membranes. BcActA and EF1b are the actin‐ and elongation factor‐encoding genes used as constitutively expressed genes (Gioti et al., 2006). BcBOT genes are the botrydial biosynthesis genes described in Pinedo et al. (2008). All gene and primer information is given in Table S1. The B05.10 strain was cultivated on grape juice medium and inoculated on tomato leaves. Three days post‐inoculation, total RNA was extracted, labelled with 32P and hybridized to the membranes.