Table 2.

Glucokinase expression and activity in the wild‐type and Δzfr1 strains grown on germ and endosperm fractions of maize kernels.

Fungal strain	HXK1 expression*		HXK2 expression		Glucokinase activity†
	Germ	Endosperm	Germ	Endosperm	Germ	Endosperm
Wild‐type	1.0 (0.7–1.3)	120 (100–150)	1.0 (0.7–1.3)	27.3 (27.0–27.7)	2.0 ± 0.2	131 ± 4.9
Δzfr1	1.5 (1.3–1.9)	45 (40–50)	–0.7 (–0.3 to –1.1)	18.2 (18.0–18.3)	1.7 ± 0.4	119 ± 3.7

^* As analysed by qPCR. Expression of HXK1 and HXK2 was normalized to TUB2 and was calculated relative to expression in wild‐type growing on germ tissue. Data represent fold differences in expression. The relative expression of each gene was calculated as 2^ΔΔCt. The range of expression, in parentheses, for each gene = (2^ΔΔCt–s–2^ΔΔCt+s), where s = the standard deviation of the ΔΔCt value.

^† Data are reported as 1 × 10⁻⁴ units of glucokinase activity per gram total protein ± the standard error of the mean for three samples.