Table 1.
Primer sequences for eight candidate reference genes and real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) amplification efficiency parameters.
| Gene name | Gene symbol | GenBank Acc. | Primer sequence | Amplicon length (bp) | E (%)* | R 2 † | |
|---|---|---|---|---|---|---|---|
| Actin | ACT | BT013707 | For | 5′‐AGGCAGGATTTGCTGGTGATGATGCT‐3′ | 107 | 101.5 | 0.998 |
| Rev | 5′‐ATACGCATCCTTCTGTCCCATTCCGA‐3′ | ||||||
| Cyclophilin (peptidyl‐prolyl cis–trans isomerase) | CyP | AK326854 (TC174635)‡ | For | 5′‐CTCTTCGCCGATACCACTCC‐3′ | 120 | 99.4 | 0.998 |
| Rev | 5′‐TCACACGGTGGAAGGTTGAG‐3′ | ||||||
| Elongation factor 1‐α | EF1α | X53043 | For | 5′‐ATTGGAAATGGATATGCTCCA‐3′ | 100 | 98.6 | 0.998 |
| Rev | 5′‐TCCTTACCTGAACGCCTGTCA‐3′ | ||||||
| Glyceraldehyde 3‐phosphate dehydrogenase | GAPDH | U93208 | For | 5′‐ACCACAAATTGCCTTGCTCCCTTG‐3′ | 110 | 97.6 | 0.998 |
| Rev | 5′‐ATCAACGGTCTTCTGAGTGGCTGT‐3′ | ||||||
| β‐Tubulin | TUB | DQ205342 | For | 5′‐CCTGACAGCTTCTGCCATGT‐3′ | 106 | 97.9 | 0.998 |
| Rev | 5′‐CATCTTCAGCCCAGTTGGTG‐3′ | ||||||
| Ubiquitin 3 | UBI | X58253 | For | 5′‐TCGTAAGGAGTGCCCTAATGCTGA‐3′ | 119 | 103.2 | 0.998 |
| Rev | 5′‐CAATCGCCTCCAGCCTTGTTGTAA‐3′ | ||||||
| Uridylate kinase | UK | AK322232 (TC196198)‡ | For | 5′‐TGGTAAGGGCACCCAATGTGCTAA‐3′ | 114 | 99.6 | 0.998 |
| Rev | 5′‐ATCATCGTCCCATTCTCGGAACCA‐3′ | ||||||
| 18S rRNA | 18S | X51576 | For | 5′‐GGGCATTCGTATTTCATAGTCAGA‐3′ | 98 | 98.2 | 0.998 |
| Rev | 5′‐GTTCTTGATTAATGAAAACATCCT‐3′ | ||||||
*
Measure of the real‐time PCR efficiency (calculated by the standard curve method).
†
Regression coefficient calculated from the regression line in the standard curve.
‡
Corresponding accession number from DFCI Tomato Gene Index.