Table 2.
Descriptive statistics of eight candidate reference genes based on their cycle threshold values as calculated by BestKeeper.
| Ranking: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
|---|---|---|---|---|---|---|---|---|---|
| Leaves (n= 24) | Gene name | ACT | UK | GAPDH | UBI | TUB | CyP | 18S | EF1α |
| Geo Mean [Ct] | 21.73 | 24.38 | 22.59 | 22.20 | 23.35 | 20.01 | 24.68 | 26.65 | |
| Min. max [Ct] | 20.92, 22.95 | 23.38, 25.07 | 21.54, 23.80 | 20.65, 23.91 | 21.77, 25.24 | 17.90, 23.96 | 19.21, 27.91 | 22.87, 36.45 | |
| Std dev [±Ct] | 0.37 | 0.45 | 0.46 | 0.57 | 0.60 | 1.40 | 2.05 | 2.44 | |
| Min. max [x‐fold] | −1.75, 2.33 | −1.99, 1.61 | −2.07, 2.31 | −2.91, 3.28 | −2.98, 3.71 | −4.31, 15.45 | −44.52, 9.39 | −13.76, 889.32 | |
| Std dev [±x‐fold] | 1.29 | 1.37 | 1.38 | 1.49 | 1.52 | 2.64 | 4.15 | 5.42 | |
| Roots (n= 15) | Gene name | UBI | UK | ACT | CyP | GAPDH | TUB | 18S | EF1α |
| Geo Mean [Ct] | 20.40 | 23.59 | 20.80 | 19.29 | 22.29 | 22.18 | 26.97 | 29.22 | |
| Min. max [Ct] | 18.84, 22.18 | 22.02, 25.50 | 18.83, 23.17 | 17.70, 21.41 | 20.19, 25.07 | 19.76, 24.95 | 23.81, 34.16 | 26.70, 33.36 | |
| Std dev [±Ct] | 0.89 | 1.03 | 1.06 | 1.07 | 1.12 | 1.32 | 1.75 | 2.02 | |
| Min. max [x‐fold] | −2.95, 3.43 | −2.98, 3.75 | −3.90, 5.19 | −3.02, 4.35 | −4.27, 6.89 | −5.35, 6.83 | −8.93, 145.91 | −5.71, 17.72 | |
| Std dev [±x‐fold] | 1.85 | 2.05 | 2.08 | 2.10 | 2.17 | 2.50 | 3.37 | 4.04 | |
| All combined (n= 39) | Gene name | GAPDH | ACT | UK | UBI | TUB | CyP | 18S | EF1α |
| Geo Mean [Ct] | 22.47 | 21.36 | 24.07 | 21.49 | 22.89 | 19.73 | 25.54 | 27.61 | |
| Min. max [Ct] | 20.19, 25.07 | 18.83, 23.17 | 22.02, 25.50 | 18.84, 23.91 | 19.76, 24.95 | 17.70, 23.96 | 19.21, 34.16 | 22.87, 36.45 | |
| Std dev [±Ct] | 0.72 | 0.76 | 0.76 | 0.93 | 0.98 | 1.23 | 1.98 | 2.49 | |
| Min. max [x‐fold] | −4.87, 6.05 | −5.78, 3.50 | −4.15, 2.69 | −8.77, 5.10 | −6.26, 5.35 | −4.09, 18.74 | −80.57, 393.51 | −26.73, 457.84 | |
| Std dev [±x‐fold] | 1.65 | 1.69 | 1.69 | 1.90 | 1.97 | 2.35 | 3.94 | 5.62 | |
[Ct], cycle threshold; Geo Mean [Ct], geometric mean [Ct]; Min, max [Ct], extreme values of Ct; Min, max [x‐fold], extreme values of expression levels expressed as absolute x‐fold over or under coefficient; n, number of samples; Std dev [±Ct], standard deviation [Ct]; Std dev [±x‐fold], standard deviation of the absolute regulation coefficients.