Figure 3.

(A) Northern blot hybridization of the 3′p23+3′UTR transgene‐derived transcripts of non‐inoculated antisense (AS, lanes 10, 16 and 24), sense (S, lanes 12 and 38), intron‐hairpin (ihp, lanes 6 and 34) and empty vector (EV) lines. Total RNA was separated by electrophoresis in a formaldehyde‐agarose gel, transferred to a nylon membrane and probed with a digoxigenin‐labelled DNA from the 3′p23+3′UTR construct. (B) Analysis of siRNAs was performed by hybridization with a radioactively labelled 3′p23+3′UTR riboprobe for detecting the plus strand, digestion with RNase, separation of the resistant fragments by denaturing polyacrylamide gel electrophoresis (PAGE) in a 12% gel, dehydration and autoradiography. Lane M corresponds to radiolabelled DNA markers of 15–26 nucleotides. Lane C corresponds to the riboprobe digested with RNase. Equal RNA load was assessed by gel electrophoresis and ethidium bromide staining (bottom panels).