Figure 4.

Accumulation of siRNAs in non‐inoculated Mexican lime transformed with the intron‐hairpin (ihp) 3′p23+3′UTR construct, the propagations of which resulted in susceptibility (S) or resistance (R) to Citrus tristeza virus (CTV) challenge. EV and (EV + CTV) correspond to non‐inoculated and CTV‐inoculated controls transformed with the empty vector, respectively. siRNAs were detected by a RNase protection assay, as in Fig. 3B. A RNase‐resistant fragment of approximately 15 nucleotides present in all preparations indicates similar RNA loading.