Table 1.

Properties of Mexican lime lines transformed with an intron‐hairpin construct derived from the 3′‐terminal 549 nucleotides of Citrus tristeza virus (CTV) RNA.

	Line	Number of transgene loci	Transgene integrity	Transcript accumulation*	siRNA accumulation*	Resistant/total propagations
Resistant	1	1	Yes	+/−	+	2/6
	20	2	No	++	ND	1/6
	27	1–2†	Yes	+/−	ND	3/10
	28	1	Yes	+	++	1/6
	29	1	Yes	+	+++	5/9
	39	1	Yes	+	++	4/10
	43	2	No	+/−	ND	2/9
	46	1	Yes	++	++++	2/11
Susceptible	5	3–4†	Yes	+/−	ND	0/6
	6	2	Yes	+	+++	0/11
	12	1	Yes	+/++	+/++	0/6
	18	1–2†	Yes	+	ND	0/6
	21	2	Yes	−	ND	0/6
	22	2	No	++	ND	0/6
	23	3	Yes	−	ND	0/6
	25	1	Yes	++	+/++	0/6
	26	2	Yes	+++++	+++	0/11
	30	2	No	−	ND	0/6
	31	4	Yes	+++	ND	0/8
	32	4	Yes	−	ND	0/6
	34	3	Yes	++	+++	0/8
	35	2	Yes	−	ND	0/6
	38	5–7†	Yes	−	ND	0/6
	41	2	Yes	++	ND	0/6
	42	1	Yes	+++	++++	0/6
	45	2	Yes	++	ND	0/6
	48	2	No	++++	ND	0/6
	51	1	Yes	+++	+++	0/6
	52	1	Yes	+++	+++	0/6
Control	C1	0		—	—	0/11
	C2	0		—	—	0/10

^* Relative accumulation from undetectable (−) to strong (+++++) signals. ND, not determined.

^† The number of hybridization bands varied depending on the restriction enzyme (ApaI or NheI) used for the Southern blot analysis of genomic DNA.