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. 2010 Jan 24;11(3):371–381. doi: 10.1111/j.1364-3703.2010.00610.x

Figure 2.

Figure 2

Elicitation of the hypersensitive response (HR) in tobacco leaves by a variety of treatments. (A) Treatments of purified PopW and cell‐free lysate preparations (CLPs) of Escherichia coli BL21 (DE3): 1, PopW (0.1 mg/mL); 2, heat‐treated PopW (0.1 mg/mL); 3, proteinase K‐treated PopW (0.1 mg/mL); 4, CLP of E. coli BL21(DE3) harbouring harpin domain of PopW; 5, CLP of E. coli BL21(DE3) harbouring PL domain of PopW; 6, CLP of E. coli BL21(DE3) harbouring control vector. (B) Suspensions of popW mutant ZJ3722 and wild‐type Ralstonia solanacearum ZJ3721: 7, bacterial suspension of wild‐type R. solanacearum ZJ3721 (107 colony‐forming units (cfu)/mL); 8, bacterial suspension of popW mutant ZJ3722 (107 cfu/mL); 9, sterile water control. (A) and (B) were taken 24 and 48 h after infiltration, respectively.