Figure 5.

Sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) showing PopW specifically binding to calcium pectate. Heat‐treated (100 °C for 10 min) PopW protein was used for the following assays: (A) PopW binding to calcium pectate beads; (B) PopW binding to calcium alginate beads; (C) PopW binding to calcium chloride; (D) PopW binding to soluble pectate. Lanes: 1, the original protein–bead mix; 2, the supernatant of the centrifuged protein–bead mix; 3, the washed beads treated with ethylenediaminetetraacetic acid (EDTA); 4, the final buffer wash; 5, PopW mixed with CaCl₂ (C) or with soluble pectate (D); 6, the supernatant of the centrifuged mix; 7, the pellet of the centrifuged mix.