Table 1.
Strains, plasmids and gene targets used in this study.
| Strain, plasmid | Relevant information* | Reference or source |
|---|---|---|
| Strain | ||
| Escherichia coli DH5α | F‐Φ80dlacZΔM15 recA1 endA1 gyrA96 thi‐1 hsdR17 (rK‐ mK +)supE44 relA1 deoRΔ(lacZYA‐argF)U169 | Gibco‐BRL |
| BL21(DE3) | F‐ ompT hsdSB (rB ‐ mB ‐) gal dcm (DE3) | Novagen |
| BL159 | BL21(DE3) express protein of PopW(1–159) | This study |
| BL366 | BL21(DE3) express protein of PopW(160–366) | This study |
| Agrobacterium tumefaciens LBA4404 | Wild‐type, Rifr, Smr | Hoekema et al., 1983 |
| Ralstonia solanacearum ZJ3721 | Wild‐type isolated from Ipomoea batatas, Zhejiang, China | This study |
| R. solanacearum ZJ3722 | popW‐deficient mutant of ZJ3721 | This study |
| R. solanacearum ZJ3723 | hrpB‐deficient mutant of ZJ3721 | This study |
| R. solanacearum ZJ3724 | hrpV‐deficient mutant of ZJ3721 | This study |
| Plasmid | ||
| pMD19‐T Simple | T‐A cloning vector for polymerase chain reaction (PCR) fragments, Apr | TaKaRa |
| pET30a(+) | T7 promoter‐based expression vector; Kmr | Novagen |
| pETpopW | 1140 bp NdeI and HindIII fragment of popW in pET30a(+) | This study |
| pETpopW(1–159) | popW(1–159) in pET30a(+) | This study |
| pETpopW(160–366) | popW(160–366) in pET30a(+) | This study |
| pTOK2 | Narrow host‐range cloning vector, Tcr | Kitten and Willis, 1996 |
| pTOKpopW′ | 449 bp HindIII and BamHI fragment of interal popW in pTOK2 | This study |
| pTOKhrpB′ | 489 bp HindIII and BamHI fragment of interal hrpB in pTOK2 | This study |
| pTOKhrpV′ | 599 bp HindIII and BamHI fragment of interal hrpV in pTOK2 | This study |
| pBI121‐GFP | Plant expression binary vector, Kmr | Andreeva and Kutuzov, 2001 |
*
Apr, Kmr, Tcr, Rmr and Smr indicate ampicillin‐, kanamycin‐, tetracycline‐, rifampicin‐ and streptomycin‐resistant, respectively.
Only the host and origin of Ralstonia solanacearum strains were provided.