Table 2.
Primers used in this study.
| Gene target | Primer sequence (5′ → 3′)* | Restriction site added |
|---|---|---|
| popW | popW F: CGCATATGTCCATCCAGATTGATCGC | NdeI |
| popW R: GCAAGCTTGCCCGAGTAGGCCTTGTAG | HindIII | |
| popW (1–159) | popW(1–159) F: ATAAGCTTGGTCGGCTCGGGCGGCTT | HindIII |
| popW(1–159) R: GCCATATGTCCATCCAGATTGATCGCCCG | NdeI | |
| popW (160–366) | popW(160–366) F: GCAAGCTTCAGCCCATCGAAGGGCGCATC | HindIII |
| popW(160–366) R: GCCATATGGGCGTGGTCGACGTCAGCAAG | NdeI | |
| popW′ | popW′ F: GCGGATCCCCACGGCGGCGGTCATCAT | BamHI |
| popW′ R: CGAAGCTTCGTCGCCGCCGG A ATACTGC | HindIII | |
| hrpB′ | hrpB′ F: GCGGATCCATTTCGACCGTGCGTATCAGC | BamHI |
| hrpB′ R: GCAAGCTTTCGAGCAGGCGGTTGGAGCAG | HindIII | |
| hrpV′ | hrpV′ F: GCAAGCTTGCGGACGAGCCGACCACCGACAG | HindIII |
| hrpV′ R: GCGGATCCGCGCTGAGCAGCATCAAGGGCAGCA | BamHI | |
| popW″ | popW″ F: TCTAGAATGTCCATCCAGATTGATCGC | XbaI |
| popW″ R: GGATCCGCCCGAGTAGGCCTTGTAGCT | BamHI |
*
Italic letters show restriction endonuclease recognition sequences. ‘F’ and ‘R’ denote forward and reverse primers of the genes, respectively.