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Virus‐induced gene silencing of NbARF1 in Nicotiana benthamiana. The two youngest leaves of a 4‐leaf stage N. benthamiana plantlet were infiltrated with pTV:ARF1 or with pTV:00 as a control treatment. Observations were done 4 weeks after inoculation. (a) Growth of ARF1 silenced plants (pTV:ARF1, right) was stunted compared to control plants (pTV:00, left). (b) NbARF1 transcript level was reduced by 80% in ARF1 silenced plants compared to control plants as quantified by real time PCR.
