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The frequencies of correctly targeted gene replacement strains at the MgTUB1 and MgERG11 loci, generated in the wild‐type and ΔMgKU70 parental strains.
| Gene | Parent strain | Ectopic transformants | Gene replacement transformants |
|---|---|---|---|
| MgTUB1 | Wild‐type | 13/46 | 33/46 |
| 28.3% | 71.7% | ||
| MgTUB1 | ΔMgKU70 | 1/46 | 45/46 |
| 2.2% | 97.8% | ||
| MgERG11 | Wild‐type | 63/129 | 66/129 |
| 48.8% | 51.2% | ||
| MgERG11 | ΔMgKU70 | 1/88 | 87/88 |
| 1.1% | 98.9% |
