Comparison of mutation frequency (mutfr), normalized Shannon index (normH) and positive‐selection site frequency (PSfr) between viral populations issued from clone SON41p and from the cylindrical inclusion (CI) chimera into Nicotiana spp. (inocula), the resistant pepper HD285 and the susceptible pepper Yolo Wonder based on high‐throughput sequencing of the central part of the genome‐linked viral protein (VPg) cistron (nucleotide positions 5930–6168); 1577–2407 sequences were analysed for each sample. The Kruskal–Wallis chi‐squared test (KW) and corresponding significance (P) are indicated for each comparison between viruses.