Table 2.

Starting amount of RNA and subsequent yields after linear amplification.

Starting RNA for the 1st round (ng)	1st round amplification (ng/µL)	Starting RNA for the 2nd round (ng)	2nd round amplification (ng/µL)
1 ng TRT	8.8	440	26.6
5 ng TRT	8.0	400	97.3
10 ng TRT	8.9	445	616.3
250 ng TRT	437.4	–	–
150 LCM GCs	14.2	720	930
200 LCM CCs	25.9	167	766
10 ng galls, 1 dpi	148.2	1482	2725
10 ng control, 1 dpi	136.5	1365	2578

LCM CCs, laser‐capture‐microdissected control cells from root vascular tissue; LCM GCs, laser‐capture‐microdissected giant cells at 3 days post‐inoculation; TRT, tomato root tissue.

All samples were subjected to two rounds of T7‐based linear RNA amplification, except for the 250‐ng input sample.