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. 2007 Nov 23;9(1):59–66. doi: 10.1111/j.1364-3703.2007.00448.x

Figure 2.

Figure 2

Northern blots resolving the expression pattern of individual genes. (a) Schematic representation of rust infection structures. (b) Ethidium‐bromide‐stained denaturing agarose gel (loading control). (c–e) Northern blots for Uf‐HSP42 (c), Uf‐HSP40 = Uf‐ISSP7 (d) and Uf‐ISSP2 (e). Probes were selected to show one gene that was represented in the haustorial library, one that was represented in both libraries, and one that was represented in the library of in vitro infection structures only. 1, uredospore (SP); 2, germ tube (GT) after 4 h germination; 3–6, in vitro infection structures harvested after 6 h (3, AP, appressorium), 12 h (4, SV, substomatal vesicle), 18 h (5, IH, infection hypha), 22 h (6, HM, haustorial mother cell, corresponds to the in vitro infection structures library); 7, isolated haustoria (HA), corresponds to the haustorial library; 8, infected leaves; 9, non‐infected leaves. bAP, bulk apoplast; NB, neckband; EM, extrahaustorial matrix. Numbers on the right give the size estimate in kb.