Table 1.
Name | Sequence | Used for |
---|---|---|
Random nonamers | 5′‐CGATTGAATTCTAGACCTGCCTCGAGNNNNNNNNA‐3′ | Reverse transcription |
5′‐CGATTGAATTCTAGACCTGCCTCGAGNNNNNNNNC‐3′ | ||
5′‐CGATTGAATTCTAGACCTGCCTCGAGNNNNNNNNG‐3′ | ||
5′‐CGATTGAATTCTAGACCTGCCTCGAGNNNNNNNNT‐3′ | ||
pSuc5′‐2 | 5′‐CTCGTCATTGTTCTCGTTCCC‐3′ | PCR |
pSuc5′ | 5′‐CAAGCATACAATCAACTCCAAG‐3′ | PCR and sequencing |
pSuc3′‐2 | 5′‐CTTCTCTTCTAAATACTCCTCTG‐3′ | PCR and sequencing |
pSuc3′ | 5′‐GTCAAATCATCGGAAGTAGC‐3′ | PCR |
EcoRI–NotI | 5′‐AATTCGCGGCCGC‐3′ | Adaptors |
3′‐GCGCCGGCG‐5′ |