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. 2010 Jun 22;11(6):757–767. doi: 10.1111/j.1364-3703.2010.00640.x

Figure 1.

Figure 1

Expression of monomeric and complexed pokeweed antiviral protein (PAP) in pokeweed leaves. (A) Proteins (4 µg) of total cell lysate, apoplastic fluid or protoplasts were separated by 12% sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE), transferred to nitrocellulose and probed with a polyclonal antibody specific to PAP (1:5000). (B) Total protoplast protein (8 µg) or proteins from the microsomal membrane (MM) fraction of protoplasts (8 µg) were also probed for the presence of PAP as described in (A). Samples that were boiled prior to separation are labelled B, and NB indicates samples that were not boiled. Molecular weights of monomeric (29 kDa) and complexed (58 kDa) PAP are indicated.