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. 2010 Jun 22;11(6):757–767. doi: 10.1111/j.1364-3703.2010.00640.x

Figure 2.

Figure 2

Separation of monomer from complexed pokeweed antiviral protein (PAP) by ion exchange chromatography. PAP was purified from the total cell lysate of pokeweed leaves by ion exchange. Positively charged proteins were eluted from the anionic column using an NaCl step gradient. Aliquots (10 µL) from each fraction were separated by 12% sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) without prior boiling, transferred to nitrocellulose and probed with a polyclonal antibody specific to PAP (1:5000). Purified monomeric PAP was loaded (20 ng) as a standard. Molecular weights of monomeric (29 kDa) and complexed (58 kDa) PAP are indicated.