Figure 6.
Translation inhibition by monomeric and complexed pokeweed antiviral protein (PAP). Poly(U) RNA was translated in rabbit reticulocyte lysate in the presence of increasing molar amounts of monomeric or complexed PAP and [3H]‐phenylalanine. –RNA indicates a translation reaction without mRNA and –PAP indicates a reaction without PAP. The protein products were precipitated in trichloroacetic acid (TCA) and quantified by liquid scintillation counting. Percentage incorporation refers to the amount of [3H]‐phenylalanine incorporated into protein relative to the total amount in the translation mix. Values represent means ± standard error for three independent experiments.