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. 2010 Dec 6;12(4):373–380. doi: 10.1111/j.1364-3703.2010.00679.x

Figure 5.

Figure 5

Secretion assay of Rsa1 by Western blot analysis using anti‐Rsa1: 1, cellular proteins of SL2029(pRS13); 2, culture supernatants from SL2029(pRS13); 3, cellular proteins of SL2029 rsa1::Tn3‐gusA200(pLAFR3); 4, culture supernatants from SL2029 rsa1::Tn3‐gusA200(pLAFR3); 5, cellular proteins of SL2029 rsa1::Tn3‐gusA200(pRS13); 6, culture supernatants from SL2029 rsa1::Tn3‐gusA200(pRS13); 7, cellular proteins of SL2029 hrcN::Tn3‐gusA20(pRS13); 8, culture supernatants from SL2029 hrcN::Tn3‐gusA20(pRS13). The arrow indicates Rsa1.