Figure 6.
Expression patterns of RFDD‐PCR candidate genes and other genes of interest in Beta vulgaris and Beta macrocarpa roots upon response to early BNYVV infection. Analyses were conducted on healthy (H) or infected (B) B. vulgaris root total RNAs extracted after 7 days of bioassay treatment and on healthy (h) or infected (i) B. macrocarpa root total RNAs extracted after 13 days of plant systemic infection. Semi‐quantitative RT‐PCR was standardized with the L11 ribosomal protein gene (accession no. BE590348) and the BNYVV RNA 3 sequence was used as a viral control. Signals were quantified with ImageJ 1.38w software and transcript accumulation was expressed as a ratio between infected value and non‐infected value (ratio I/H or i/h). Identifiers (no.) of RFDD‐PCR cDNAs are those indicated in Table 1.