Table 2.
Segregation of white rust resistance amongst progeny of Brassica juncea cv. Cutlass and B. napus line ACS‐N32 following transformation with the WRR4 TIR‐NB‐LRR (Toll‐like/interleukin‐1 receptor‐nucleotide binding‐leucine‐rich repeat) gene from Arabidopsis thaliana.
| Brassica species | Line no. | No. of T1 insertions | Total T2 | R | S | χ2 | P |
|---|---|---|---|---|---|---|---|
| B. juncea | 1 | 1 | 42 | 30 | 12 | 0.28 | 0.59 |
| 2 | 2 | 41 | 32 | 9 | 0.20 | 0.65 | |
| 3 | 2 | 35 | 23 | 12 | 1.60 | 0.20 | |
| 4 | 1 | 41 | 37 | 4 | 5.08 | 0.02 | |
| 5 | 4 | 9 | 9 | 0 | 3.00 | 0.08 | |
| 6 | 1 | 40 | 27 | 13 | 1.20 | 0.27 | |
| 7 | 1 | 40 | 31 | 9 | 0.13 | 0.71 | |
| 8 | 1 | 41 | 27 | 14 | 1.82 | 0.17 | |
| B. napus | 1 | 4 | 41 | 37 | 4 | 5.08 | 0.02 |
| 2 | 1 | 39 | 22 | 17 | 7.18 | 0.007 | |
| 3 | 1 | 25 | 18 | 7 | 0.12 | 0.72 |
The number of transgene insertions in the T1 generation was determined by Southern blotting. The numbers of resistant (R; green cotyledon and no rust pustules) and susceptible (S; large pustules formed profusely on the underside of cotyledons) T2 seedlings were recorded at 10 days after inoculation.