Table 4.

Target sequence (GenBank accession), primer (FP, RP) and probe (P) sequences and their optimized concentrations used in quantitative polymerase chain reactions (qPCRs) for elongation factor 1 (EF‐1), 2‐oxoglutarate‐dependent dioxygenase (2‐ODD), photosystem II protein (PS II) and glyceraldehyde‐3‐phosphate dehydrogenase (GA3PDH) genes.

Amplicon	Target sequence	Sequence (5′–3′)		Reaction conc. (nm)
EF‐1	AB061263	FP	GGAAGCTGCTGAGATGAACAAGA	900
		RP	CTCACGTTCAGCCTTAAGTTTGTC	900
		P	FAM‐TCATTCAAGTATGCCTGGGTGCT‐TAMRA	250
2‐ODD	BQ113054	FP	CGCATCATTGGAGCCCTTCAAAGTC	900
		RP	AGGTAACAAAGGTAGGATACAAACTAGAAAGACATTG	900
		P	FAM‐TTGTGTGCCAGAAGGATCAAGTCTATGCTG‐TAMRA	250
PS II	BQ111818	FP	GACCCTGCCCCTGCTACTGG	300
		RP	CTTTGTGAATCCAAAAAGTGGACCTCCTTC	300
GA3PDH	BQ113669	FP	CCAGGCCGGAGCCAAGAAGG	300
		RP	TCATGGTTGTAAAGTTCAGCATTGACACC	300
		P	FAM‐CGCCCCCGGAAAAGGTGATATCCCTACTTA‐TAMRA	150