Figure 2.

Segregation of Bean common mosaic virus (BCMV) resistance and a cleaved amplified polymorphic sequence (CAPS) marker differentiating eIF4E alleles PveIF4E² (4E²) and PveIF4E¹ (4E¹). A Phaseolus vulgaris F₂ population was generated from a cross between parents (P) USCR8 (4E²/4E², lane 2) and Dubbele Witte (DW) (4E¹/4E¹, lane 3). Successful crossing was verified on F₁ individuals (lane 4) and results from 17 F₂ individuals are shown (lanes 5–21).The response to BCMV was assayed by enzyme‐linked immunosorbent assay (ELISA). Plants with an ELISA A405 (absorbance at 405 nm) reading of more than 2.5 times that of the mock‐inoculated control were rated as susceptible (s); plants with an ELISA A405 reading of less than 2.5 times that of the mock‐inoculated control were rated as resistant (r). The genotype was determined by restriction enzyme RsaI digestion of a 541‐bp polymerase chain reaction (PCR) product amplified with primers ENM‐FWe and ENM‐RVe from genomic DNA. DNA fragments were analysed by agarose gel electrophoresis with a 100‐bp ladder as marker (lane 1).