Table 2.
List of the transcripts significantly induced and repressed by Puccinia striiformis f. sp. tritici (Pst) in reference to mock‐inoculated controls in the incompatible (Yr5) interaction and the compatible (yr5) interaction after two‐way anova (P < 0.001, treatment effect only), FC > 2.0 cutoff, and FDR correction. Values represent mean log2 FC, GO refers to gene ontology, and putative function represents the best database hit from HarvEST
| Putative GO category | Putative function | Probe set | Incompatible interaction | Compatible interaction | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 6 h | 12 h | 24 h | 48 h | 6 h | 12 h | 24 h | 48 h | |||
| HR‐specific transcripts | ||||||||||
| Defence—cell wall | Proline‐rich protein | Ta.16599.1.S1_at | 1.44 | 2.39 | ||||||
| Defence—cell wall | Pathogen‐induced protein WIR1A homologue | Ta.22732.1.S1_x_at | 2.05 | 1.62 | ||||||
| Defence—cell wall | Pathogen‐induced protein WIR1A homologue | Ta.3133.1.S1_x_at | 2.86 | 3.67 | ||||||
| Defence—oxidative burst | Peroxidase | Ta.18497.1.S1_at | 2.49 | 2.50 | ||||||
| Defence—oxidative burst | Peroxidase | Ta.24106.1.S1_x_at | 1.87 | 1.36 | ||||||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | Ta.7022.1.S1_at | 1.35 | |||||||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | TaAffx.131379.1.A1_at | 2.44 | |||||||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | TaAffx.92008.1.A1_s_at | 2.35 | 1.79 | ||||||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.1174.1.S1_x_at | 2.80 | 2.78 | ||||||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.21297.1.S1_at | 2.56 | 1.95 | ||||||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.21354.1.A1_at | 1.04 | |||||||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.21354.1.A1_x_at | 1.27 | |||||||
| Defence—PR protein | PR protein 10 | Ta.22619.1.S1_at | 1.83 | 2.98 | ||||||
| Defence—PR protein | PR protein 10 | Ta.22619.1.S1_x_at | 2.15 | 3.46 | ||||||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.26048.1.S1_x_at | 1.60 | 1.57 | ||||||
| Defence—PR protein | Thaumatin‐like protein | Ta.27762.1.S1_x_at | 1.66 | 2.71 | ||||||
| Defence—PR protein | Chitinase | Ta.30501.1.S1_at | 2.61 | 3.88 | ||||||
| Defence—PR protein | Beta‐1,3‐glucanase | TaAffx.119315.2.S1_at | 1.36 | |||||||
| Defence—PR protein | Beta‐1,3‐glucanase | TaAffx.119315.2.S1_x_at | 1.03 | 1.41 | ||||||
| Energy—electron transport | Cytochrome P450 | Ta.29826.1.S1_at | 1.43 | |||||||
| Energy—electron transport | Cytochrome P450 | Ta.8262.1.S1_at | 2.00 | 1.46 | ||||||
| Energy—electron transport | Cytochrome P450 | Ta.8447.1.S1_a_at | 1.21 | 1.96 | 5.00 | 2.00 | ||||
| Energy—electron transport | Cytochrome P450 | Ta.8447.1.S1_x_at | 3.13 | |||||||
| Energy—electron transport | Blue copper‐binding protein | Ta.9336.1.S1_x_at | 1.58 | |||||||
| Energy—electron transport | Cytochrome P450 | TaAffx.109794.1.S1_s_at | 2.86 | |||||||
| Growth—GA biosynthesis | Gibberellin oxidase | Ta.24934.3.S1_at | 1.55 | 1.91 | 1.18 | |||||
| Growth—GA biosynthesis | Ent‐kaurene synthase | Ta.8418.1.S1_at | 1.71 | 1.42 | ||||||
| Metabolism—carbohydrate | UDP‐glucose dehydrogenase | Ta.2657.1.S1_x_at | 1.08 | 2.17 | ||||||
| Metabolism—carbohydrate | SIS domain protein | Ta.4815.1.S1_at | 1.13 | 1.42 | ||||||
| Metabolism—carbohydrate | Beta‐fructofuranosidase | TaAffx.82312.1.S1_s_at | 1.10 | 1.16 | ||||||
| Signal transduction | LRR‐containing extracellular glycoprotein | Ta.27314.1.S1_at | 1.96 | 1.67 | ||||||
| Signal transduction | Secretory protein kinase | TaAffx.52945.1.S1_at | 1.45 | 2.30 | ||||||
| Transport—carbohydrate | Glucose transporter | Ta.12517.1.S1_at | 1.21 | |||||||
| Transport—carbohydrate | Sugar transporter | Ta.27329.1.S1_at | 1.34 | |||||||
| Unclear | NA | Ta.13991.1.S1_x_at | 1.15 | 2.50 | 1.51 | |||||
| Unclear | NA | Ta.14231.1.S1_x_at | 1.25 | |||||||
| Unclear | NA | TaAffx.107538.1.S1_x_at | 1.48 | |||||||
| Unclear | NA | TaAffx.110081.1.S1_at | 1.47 | 1.41 | ||||||
| Unclear | NA | TaAffx.110081.1.S1_x_at | 1.46 | 1.57 | ||||||
| Unclear | NA | TaAffx.110250.1.S1_x_at | 1.20 | 1.78 | ||||||
| Unclear | NA | TaAffx.7302.1.S1_at | 1.79 | 1.07 | ||||||
| Unknown | NA | Ta.11087.2.S1_at | 1.07 | |||||||
| Unknown | NA | Ta.11087.2.S1_x_at | 1.42 | |||||||
| Unknown | NA | Ta.14618.1.A1_at | –1.60 | |||||||
| Unknown | NA | Ta.21236.1.S1_a_at | 1.42 | 3.04 | ||||||
| Unknown | NA | Ta.21236.3.S1_x_at | 1.83 | 3.10 | ||||||
| Unknown | NA | Ta.21314.1.S1_at | 2.46 | 3.98 | 2.74 | |||||
| Unknown | NA | Ta.21314.1.S1_x_at | 2.45 | 3.83 | 2.76 | |||||
| Unknown | NA | Ta.22223.1.S1_at | 1.18 | 1.48 | ||||||
| Unknown | NA | Ta.22957.1.S1_at | 2.17 | 3.84 | 1.64 | |||||
| Unknown | NA | Ta.3247.1.S1_at | 1.04 | |||||||
| Unknown | NA | Ta.8254.1.A1_at | 2.44 | 1.18 | ||||||
| Unknown | NA | Ta.8582.1.S1_at | 1.05 | 1.88 | 3.35 | |||||
| Unknown | NA | Ta.8582.2.S1_a_at | 2.18 | |||||||
| Unknown | NA | Ta.8582.2.S1_x_at | 1.26 | 2.67 | ||||||
| Unknown | NA | TaAffx.108939.1.S1_at | 1.81 | |||||||
| Unknown | NA | TaAffx.109709.1.S1_at | 1.37 | |||||||
| Unknown | NA | TaAffx.109765.1.S1_at | 1.07 | 2.07 | ||||||
| Unknown | NA | TaAffx.27177.1.S1_at | 1.41 | |||||||
| Unknown | NA | TaAffx.52926.1.S1_at | 1.72 | 2.38 | ||||||
| Unknown | NA | TaAffx.55533.1.S1_at | 1.22 | |||||||
| Unknown | NA | TaAffx.82674.1.S1_at | 1.08 | |||||||
| Biotrophic interaction‐specific transcripts | ||||||||||
| Defence—lignin | Dirigent‐like protein | Ta.11506.1.S1_a_at | –1.56 | |||||||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | Ta.7022.1.S1_x_at | 1.34 | |||||||
| Energy—electron transport | Blue copper‐binding protein | Ta.5654.1.S1_at | 1.71 | |||||||
| Energy—electron transport | Blue copper‐binding protein | Ta.8483.1.S1_at | 1.13 | |||||||
| Metabolism—carbohydrate | Nudix hydrolase | Ta.18888.1.S1_at | 1.18 | |||||||
| Metabolism—carbohydrate | Polygalacturonase | Ta.8782.1.S1_at | 2.64 | |||||||
| Metabolism—galactose | Aldose 1‐epimerase | Ta.10680.1.S1_at | 1.41 | |||||||
| Metabolism—glycerol | Glycerophosphoryl diester phosphodiesterase | TaAffx.113645.2.S1_at | 1.19 | |||||||
| Metabolism—sterol | Cytochrome P450 51 | TaAffx.107979.1.S1_at | 3.03 | |||||||
| Metabolism—sterol | Cytochrome P450 51 | TaAffx.28047.1.S1_at | 4.54 | |||||||
| Metabolism—sterol | Cytochrome P450 51 | TaAffx.28047.1.S1_s_at | 1.27 | |||||||
| Signal transduction | Protein kinase | Ta.25487.1.S1_at | 1.22 | |||||||
| Transport | HIPL1 protein | Ta.5666.1.S1_at | 1.26 | 1.15 | ||||||
| Unclear | NA | Ta.22482.1.S1_s_at | 1.00 | |||||||
| Unclear | NA | Ta.25140.1.S1_at | 1.22 | 1.24 | 1.19 | |||||
| Unclear | NA | Ta.806.2.A1_a_at | –1.17 | |||||||
| Unclear | NA | TaAffx.53352.1.S1_x_at | 1.78 | 1.08 | ||||||
| Unclear | NA | TaAffx.78553.1.S1_at | 1.06 | |||||||
| Unknown | NA | Ta.16165.1.S1_at | 2.24 | 1.17 | ||||||
| Unknown | NA | Ta.27882.1.S1_x_at | 1.17 | |||||||
| Unknown | NA | TaAffx.7236.1.S1_at | 1.05 | 2.63 | ||||||
| Basal defence transcripts | ||||||||||
| Defence | Pathogen‐induced secretory protein | Ta.231.1.S1_at | 1.29 | 2.03 | ||||||
| Defence | Pathogen‐induced secretory protein | Ta.231.1.S1_x_at | 1.71 | 2.58 | ||||||
| Defence—alkaloid | Reticuline oxidase | Ta.27350.1.S1_at | 1.59 | 1.46 | 1.32 | 2.00 | ||||
| Defence—anthocyanin | UDP‐glycosyltransferase | Ta.23340.1.S1_at | 2.55 | 5.17 | 3.85 | 1.41 | 5.28 | 2.79 | ||
| Defence—anthocyanin | UDP‐glycosyltransferase | Ta.30731.1.S1_at | 1.69 | 4.89 | 3.47 | 5.33 | 2.26 | |||
| Defence—lignin | Dirigent‐like protein | Ta.22687.1.A1_at | 1.13 | 2.24 | 1.40 | 2.47 | ||||
| Defence—lignin | Cinnamyl‐alcohol dehydrogenase | Ta.28562.1.A1_at | 1.09 | 2.38 | 2.89 | |||||
| Defence—lignin | Caffeic acid 3‐O‐ methyltransferase | Ta.336.1.S1_x_at | 2.05 | 2.10 | ||||||
| Defence—lignin | Caffeic acid 3‐O‐ methyltransferase | Ta.336.2.S1_at | 1.75 | 2.05 | ||||||
| Defence—lignin | Dirigent‐like protein | TaAffx.28302.2.S1_at | 1.44 | 3.01 | 1.98 | 3.31 | ||||
| Defence—oxidative burst | Peroxidase | Ta.22564.1.S1_at | 4.01 | 1.36 | 4.49 | 1.08 | ||||
| Defence—oxidative burst | Peroxidase | Ta.22564.2.S1_a_at | 3.82 | 1.20 | 4.22 | 1.12 | ||||
| Defence—oxidative burst | Peroxidase | Ta.22564.2.S1_x_at | 3.34 | 1.09 | 3.86 | |||||
| Defence—oxidative burst | Peroxidase | Ta.5385.1.S1_at | 1.21 | 3.34 | 3.81 | 1.13 | 1.38 | 4.06 | 3.41 | |
| Defence—oxidative burst | Peroxidase | Ta.82.1.S1_at | 2.12 | 4.61 | 2.38 | 4.47 | 1.96 | |||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | Ta.1465.1.S1_at | 1.80 | 2.51 | ||||||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | Ta.7022.1.S1_s_at | 1.07 | 2.91 | 1.90 | 1.55 | 2.19 | |||
| Defence—phenylpropanoid | Phenylalanine ammonia‐lyase | Ta.9220.1.S1_a_at | 3.01 | 2.61 | 1.27 | 1.72 | 3.11 | |||
| Defence—PR protein | PR protein 1 | Ta.13013.2.S1_x_at | 1.27 | 1.10 | 1.24 | |||||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.20750.1.S1_at | 1.87 | 2.34 | 1.50 | 1.46 | 2.49 | |||
| Defence—PR protein | Beta‐1,3‐glucanase | Ta.22562.1.S1_at | 1.61 | 2.05 | 3.61 | 3.03 | 1.86 | 1.79 | 3.32 | 2.14 |
| Defence—PR protein | Beta‐1,3‐glucanase | TaAffx.131249.1.S1_at | 2.14 | 2.14 | 1.31 | 1.75 | 2.63 | 1.01 | ||
| Defence—PR protein | Beta‐1,3‐glucanase | TaAffx.131249.1.S1_s_at | 1.22 | 2.14 | 2.62 | 1.56 | 1.50 | 1.81 | 2.96 | 1.12 |
| Energy—electron transport | Cytochrome P450 | TaAffx.107507.1.S1_at | 3.64 | 4.19 | ||||||
| Energy—electron transport | Blue copper‐binding protein | TaAffx.55612.1.S1_at | 1.09 | 1.61 | 1.58 | |||||
| Metabolism—sterol | Cytochrome P450 51 | Ta.8346.1.A1_at | 1.03 | 1.32 | 2.23 | |||||
| Signal transduction | Protein kinase | Ta.27258.1.S1_at | 1.80 | 1.23 | 1.85 | |||||
| Signal transduction | Protein kinase | TaAffx.23165.2.S1_at | 1.56 | 1.70 | ||||||
| Signal transduction | Protein kinase | TaAffx.23205.1.S1_at | 1.41 | 1.84 | ||||||
| Signal transduction | Secretory protein kinase | TaAffx.52945.3.S1_at | 1.18 | 1.55 | ||||||
| Signal transduction | Secretory protein kinase | TaAffx.52945.3.S1_x_at | 1.28 | 2.06 | 2.15 | |||||
| Transport | Phosphate transporter | Ta.10084.1.S1_at | 1.70 | 1.67 | ||||||
| Transport | Sugar transporter | Ta.1760.1.S1_at | 1.13 | 1.83 | 1.93 | |||||
| Transport | Sugar transporter | Ta.1760.1.S1_x_at | 1.07 | 1.86 | 1.93 | |||||
| Transport | Amino acid permease | Ta.22300.1.S1_at | 1.46 | 2.35 | 1.27 | 3.09 | ||||
| Transport | Agmatine coumaroyltransferase | Ta.8228.1.S1_at | 1.55 | 3.03 | ||||||
| Transport | Peptide transporter | Ta.8459.1.A1_at | 2.17 | 1.88 | 1.73 | 2.52 | ||||
| Unclear | NA | TaAffx.109424.1.S1_at | 2.47 | 3.51 | 1.02 | 1.59 | 3.21 | |||
| Unknown | NA | Ta.10589.1.S1_at | 1.35 | 2.49 | 1.02 | 3.09 | ||||
| Unknown | NA | Ta.12795.1.S1_at | 1.15 | 1.56 | 2.23 | 1.23 | 1.75 | |||
| Unknown | NA | Ta.1454.3.S1_at | 1.37 | 1.07 | 1.65 | |||||
| Unknown | NA | Ta.16472.1.S1_s_at | 1.72 | 2.20 | 1.46 | 2.62 | ||||
| Unknown | NA | Ta.16472.1.S1_x_at | 1.04 | 1.88 | ||||||
| Unknown | NA | Ta.21236.2.S1_x_at | 1.81 | 2.90 | 1.01 | 1.58 | 3.05 | |||
| Unknown | NA | Ta.23165.2.S1_x_at | 1.23 | 2.10 | 1.24 | 1.58 | 1.23 | |||
| Unknown | NA | Ta.23165.3.S1_x_at | 1.25 | 1.89 | 1.21 | 1.47 | 1.11 | |||
| Unknown | NA | Ta.23340.2.S1_at | 2.33 | 5.00 | 3.06 | 1.40 | 4.97 | 2.46 | ||
| Unknown | NA | Ta.27882.1.S1_s_at | 1.49 | 1.14 | 1.54 | |||||
| Unknown | NA | Ta.30829.1.S1_at | 1.22 | 1.85 | ||||||
| Unknown | NA | TaAffx.104648.1.S1_at | 1.34 | 3.23 | 4.25 | 1.80 | 1.23 | 2.82 | 3.82 | |
| Unknown | NA | TaAffx.26815.1.S1_at | 1.55 | 1.81 | 2.04 | |||||
| Unknown | NA | TaAffx.32249.1.S1_at | 3.92 | 3.64 | 4.42 | 2.94 | 5.20 | 5.52 | 5.27 | 4.16 |
| Unknown | NA | TaAffx.53805.1.S1_at | 1.19 | 1.92 | ||||||
| Unknown | NA | TaAffx.59551.1.S1_at | 2.56 | 2.73 | 2.78 | 1.43 | 3.36 | 3.88 | 3.26 | 1.89 |