Table 3.
List of the Puccinia striiformis f. sp. tritici (Pst)‐specific Yr5 and yr5 transcripts identified as part of the constitutive Yr5‐controlled transcripts category, following two‐way anova (P < 0.001, genotype effect only), FC > 2.0 cutoff, and FDR correction. Values represent mean log2 FC of Yr5 in reference to yr5 such that positive values indicate probe sets expressed at a higher level in Yr5 and negative values are those at a higher level in yr5 samples. GO refers to gene ontology, and putative function represents the best database hit from HarvEST.
| Putative GO category | Putative function | Probe set | Mean log2 FC | |||
|---|---|---|---|---|---|---|
| 6 h | 12 h | 24 h | 48 h | |||
| Pst‐specific Yr5 transcripts | ||||||
| DNA catabolism | S1/P1 Nuclease | Ta.5497.1.A1_at | 1.19 | |||
| DNA catabolism | S1/P1 Nuclease | TaAffx.128682.1.S1_at | 1.45 | |||
| Growth—cell replication | Chromosome condensation factor | Ta.2933.2.S1_x_at | 1.18 | |||
| Signal transduction | Calmodulin‐binding protein | Ta.23807.10.S1_at | 1.03 | 1.05 | ||
| Signal transduction | Calcium‐binding EF hand protein | Ta.4155.1.S1_s_at | 1.18 | |||
| Transcription | Myb transcription factor | Ta.25545.1.S1_at | 1.42 | |||
| Unclear | NA | Ta.30565.1.A1_x_at | 1.07 | |||
| Unclear | NA | Ta.9906.3.A1_a_at | 1.03 | |||
| Unclear | NA | TaAffx.24957.1.S1_at | 1.13 | |||
| Unknown | NA | TaAffx.111269.1.S1_at | 1.25 | |||
| Unknown | NA | TaAffx.84234.1.S1_x_at | 1.27 | |||
| Unknown | NA | TaAffx.9566.1.S1_at | 1.60 | 1.38 | ||
| Pst‐specific yr5 transcripts | ||||||
| Defence | Subtilisin‐chymotrypsin inhibitor 2 | Ta.14230.1.S1_at | –1.44 | –2.09 | ||
| Defence—R protein | NB‐ARC domain containing protein | Ta.25981.1.A1_at | –1.32 | |||
| Metabolism | Inorganic pyrophosphatase | Ta.9458.2.S1_at | –1.07 | –3.26 | –3.57 | –1.40 |
| Metabolism—protein | F‐box family protein | TaAffx.59384.1.S1_at | –1.01 | |||
| Signal transduction | GTP1/OBG family protein | Ta.16040.1.A1_x_at | –1.04 | –1.60 | ||
| Signal transduction | Protein kinase | Ta.26120.1.S1_at | –1.09 | |||
| Signal transduction | Protein kinase | Ta.26120.1.S1_x_at | –1.12 | |||
| Transcription | MYND finger family protein | TaAffx.129136.1.S1_at | –1.15 | –1.41 | –1.29 | |
| Transport | Agmatine coumaroyltransferase | Ta.8228.1.S1_at | –2.18 | |||
| Unclear | NA | TaAffx.128510.8.A1_at | –1.17 | –1.16 | ||
| Unknown | NA | Ta.21783.1.S1_at | –1.04 | |||
| Unknown | NA | Ta.9866.1.S1_at | –1.06 | |||
| Unknown | NA | TaAffx.27427.1.S1_at | –1.02 | –1.21 | ||
| Unknown | NA | TaAffx.31781.1.S1_at | –1.37 | |||
| Unknown | NA | TaAffx.94040.1.A1_at | –1.09 | |||