Table 5.
Sequences of oligonucleotides used in this study.
| Primer pair | Sequence of top strand primer (5′ to 3′) | Sequence of bottom strand primer (5′ to 3′) | Product length (bp) |
|---|---|---|---|
| XhcPP01 | TTGCGGCCGGCAAAATGCAC | CTACGATCAGGCGGCGCAGG | 1323 |
| XhcPP02 | ACGCAGGCAGACACGACACG | GCGCTTTCGCTCAATGGCGG | 1041 |
| XhcPP03 | TGGGTGCCATAGCGTTGCGG | TGCGCCTCTGGTTGCACTCG | 1226 |
| XhcPP04 | CTCCACGCGCAGGTCCAGTG | GAGAAGCCTGGCTGACGCCG | 620 |
| XhcPP05 | ACAGGCCGAGTCGCAACAGC | TGCTGCCGCGAAACCCGATT | 1119 |
| XhcPP06 | AATGGATGTGGCCGCACGGG | GGTTGCGCTGGATGCGGTCT | 963 |
| XhcPP07 | AGATCGATGCGCTCGGCAGC | TTCCGACGCCGTCACCTTGC | 1405 |
| XhcPP08 | GCGCATCCATTTGCCAGCCG | CCCGCTCTTGCTCACCTGCC | 875 |
| XhcPP09 | GTTGCTTGGCGTGCGTGGTG | CGCGTGGTGGGAGCGTTCTT | 1038 |
| XhcPP10 | AGCTGTTGCCGGAACTCGCC | GCGCAGACCACGAAGTCGCT | 1207 |
| XhcPP11 | GCTGGGCTCGTCGGCGTATC | GGGAATGCCGCGTTGGTGGA | 1224 |
| XhcPP12 | TAGCTGTTGCTGCACGGCCC | TCGTTGCGCCCTCGTTGTCC | 1437 |
| XhcPP13 | AGCGGCAGCCGAGAACAACC | GCGCGCGCTACGAGATGAGT | 517 |
| XhcPP14 | ATCGGCCTGTGCAACGGTGG | ACGCGCTGCGCTGAAGAGTT | 651 |
| XhcPP15 | CATTGCGCGCATACACCGCC | CGTTGGCGCAGGTGGGGATT | 552 |
| XhcPP16 | TGTCGAACAGCCGCCCGAAC | CAGCAGTGCGGACACGCAGA | 979 |
| XhcPP17 | TCGGGCACTTGAAGGCGCAG | GTCGGTCGCGCCGTAGATGG | 523 |
| XhcPP18 | AACTCGCGCGTTCTTGCGGA | TGGCGCAACGGGGATTGGTC | 666 |
| 9B* | CATTCCAAGAAGCAGCCA | TCGCTCTTAACACCGTCA | 900 |
| 3S* | TGCCTGGCTACGGAATTA | ATCCACATCCGCAACCAT | 300 |
| XopQ | CAAAAAAGCAGGCTCCATGGATTCCATCAGGCATCGCCCC | GAAAGCTGGGTGTTTTTCAGAAGCAAGCGCCAC | 1379 |
| AvrBs2 | CAAAAAAGCAGGCTCCATGCGTATTGGTCCTTTGCAACC | GAAAGCTGGGTGCTGCTCCGGCTCGATCTGTTTGGC | 2157 |
| XopX | AGCTTGGTCGCATGTTCACC | TCTGCGAAACAGAGCATTGG | 828 |
| XopR | CATTGACGGCAGCTGCTTGC | ATAACGATGCGATACAGCG | 666 |
| HrcC, hpa1, hpa2 | ATACCGATCAGACCGATCTG | GGCAATCCGCGATGTATCC | 600 |
| B1 and B2 | GGGGACAAGTTTGTACAAAAAAGCAGGCT | AGATTGGGGACCACTTTGTACAAGAAAGCTGGGT | Gateway cloning |