Protoemic confirmation of pathogenesis‐related protein 10 (PR10) levels in RNAi‐silenced transgenic maize kernels compared with non‐silenced control (HT40917‐1) kernels. Sections of two‐dimensional gels showing the level of PR10 protein (spot ‘d’, indicated with a circle) in the non‐silenced control line HT40917‐1 (A) compared with other transgenic lines (B, A44S1‐15‐3; C, A44S1‐1‐1; D, A44S1‐3‐1; E, A44S1‐3‐4; F, A44S1‐5‐2; G, A44S1‐5‐3; H, A44S1‐6‐1; I, A44S1‐35‐3; J, A44S1‐35‐4; K, A44S1‐36‐1; L, A44S1‐42‐1). Other proteins showing changes in their levels are labelled with letters (a, starch synthase; b, ADP‐glucoase pyrophosphorylase small subunit; c, a 40S ribosomal protein and a nucleic acid‐binding protein; e, PR10 and glyoxalase; f, unknown).