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. 2011 Mar 29;12(8):786–798. doi: 10.1111/j.1364-3703.2011.00712.x

Table 1.

Transcript accumulation of defence response genes in healthy tissues of 3‐week‐old Medicago truncatula R108 relative to transcripts in leaves.

Average relative expression (SD)
PR10 PR10.1 PR10.2 TLP CHS
Root 4.2 (1.0) 2.2 (0.1) 4.2 (1.0) 0.4 (0.6) 5.3 (2.2)
Leaf 1 1 1 1 1
Stem 0.4 (0.06) 1.2 (0.03) 0.5 (0.09) 0.4 (0.2) 0.4 (0.1)
Petiole 0.8 (0.3) 0.9 (0.07) 0.6 (0.2) 1.7 (1.0) 0.5 (0.1)

Relative expression (fold difference) of five genes was determined by quantitative reverse transcriptase‐polymerase chain reaction assays using actin as the endogenous reference. Values are the average and standard deviation (SD) as determined by the ΔΔC t method (n= 3). Primers used are listed in Table S3.

CHS, chalcone synthase; PR, pathogenesis response; TLP, thaumatin‐like protein.