Figure 3.

Investigation of lipopolysaccharide (LPS) mobilization in cross‐sections of Arabidopsis thaliana leaves using fluorescently labelled LPS from Salmonella minnesota (LPS_S.min.). After pressure infiltration of 100 µg/mL fluorescein‐labelled LPS_S.min., images of cross‐sections were obtained after 3 h under fluorescent light (green light filter, 435–485 nm; red light filter, 653–695 nm). (a) LPS fluorescence (green) and autofluorescence of chloroplasts (red); (b) green fluorescence of injected LPS; (c) toludine blue staining shows pectin and pectic substances (pink to purple); (d) phloroglucinol test shows lignin (red–violet); (e) autofluorescence of ferulic acid bound to lignin‐ or cutin‐containing cells (blue). Staining was performed to characterize tissue‐specific properties. CZ, cambial zone; P, phloem; X, xylem.