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Copy number and occurrence of the protease gene PRO1 in the Plasmodiophora brassicae genome. (a) Southern analysis. Genomic DNA was digested with restriction enzymes as indicated at the top of each lane and hybridized with digoxigenin‐labelled PRO1. (b) Polymerase chain reaction (PCR) amplification of a PRO1 internal fragment from DNA of P. brassicae from soil and from single‐spore isolates of pathotypes P2–P8. The primers used were F2 and R4.
