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. 2008 Feb 13;9(3):293–304. doi: 10.1111/j.1364-3703.2007.00463.x

Figure 4.

Figure 4

Ctf1 regulates expression of cut1 and lip1 in axenic culture. (A) Transcript levels of the cut1 gene determined by RT‐PCR. The indicated strains were grown for 12 h in PDB and transferred for 20 h to SM with 1% glucose or 0.2% apple cutin as carbon source. (B) Expression of cut1 determined by RT‐PCR in the indicated strains grown for 48 h in SM with 0.2% apple cutin. (C) Northern analysis for lip1 transcript accumulation. Strains were grown for 12 h in PDB and transferred for 24 h to SM with 2% wheat germ oil as carbon source. Upper panel: the filter hybridized to the lip1 probe. Middle panel: the filter hybridized to the act1 probe. Lower panel: total RNA stained on the filter with methylene blue.