Table 1.

Oligonucleotides used in this study.

Primer	Sequence
ctf1	GGAATGATAATCCGAAGGAGC
ctf2	TGGATCCATGTTCGGCTGCCAC
ctf5	TTGACTGAGGTATCTTGCCTA
ctf4	GCCATCATGTGGTCATCGG
ctf11	GGAATGATAATCCGAAGGAGC
ctf14	GTCGCAGTTGTCGGCACCA
ctf15	TTGTCATTCTCGGCAAAGTATT
ctf16	ATCCGTGGAACAGTCGCAAC
ctf17B	TATTCTGCAGGATCCATGCGACGC
ctf18B	GCGTCGCATGGATCCTGCAGAATA
ctf20X	TCTAGAATGTCGTCAGGCAGCGGCACGC
ctf22	TTCCTCCATCATTGCTGCTTCT
cut9d	GT(N)TGGAT(H)CA(R)GG(N)GT(N)GG(N)GG
cut10d	T(Y)TG(N)A(R)(R)TT(Y)TT(N)GT(R)TA(N)CC(R)AA
cut14	GACTCCAGCAATCTTCTCCC
cut19	GGCAGCATCACCCGCAACG
lip11	GTTTTACATCCAACACGGCG
lip12	ATCTGGACATAAGAGTTCAGC
lip16	TTGGCGACGATACAGAAGCG
lip18	ACGAGACGACGATTTCCTTTC

Mutations introduced in the original sequences for creation of BamHI restriction sites (B) are shown in bold. Degenerated primers are designated with a ‘d’, and use the UIPAC code: ‘N’ for A/C/T/G, ‘R’ for A/G; ‘H’ for A/T/C, and ‘Y’ for C/T.