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Secretion of HrpJ by several strains of E. amylovora under hrp gene‐inducing conditions. Strains of Ea321 (pCPP1553) indicated in the figure were grown in hrp‐inducing medium at 18 °C for 36 h. Proteins from supernatant were collected and loaded in 8% SDS‐PAGE gels; detection by Western blotting was carried out with HrpJ polyclonal antibody. WT, wild‐type.
